Why remove them from xylene?  Don't let cells and tissues dry -- ever --
unless standard protocol (e.g., air-dried blood films) calls for otherwise.
Immersing slides overnight in xylene is no different, and no more "harmful",
than coverslipping slides with xylene-based mounting medium.  The xylene
solvent remains in contact with the cells or tissue for many days until it
is able to evaporate completely but ever so slowly through the edge seal of
mounting medium that borders the cover glass.

Gary Gill

-----Original Message-----
From: Eva C Andersson [mailto:eca9@georgetown.edu] 
Sent: Friday, August 27, 2004 12:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Coverslipping?


Hi,
I have a question about coverslipping slides. Is there any harm in 
leaving slides without coverslipping them overnight? With this I mean 
after dehydration and xylenes...just removing them from the xylenes and 
letting them dry....redipping them in xylenes before coverslipping the 
following morning.
Thanks in advance for you help,
Eva

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