[Histonet] rat eyes
I am having huge problems with unperfused rat eyes. Shortly after the
eyes are enucleated I place them in oct and snap freeze the tissue in
2-methylbutane placed in liquid nitrogen. After sectioning the tissue,
I fix it for 10 min. in 4% paraformaldyhde and follow the HNE antibody
protocol. The results are that the photoreceptors are almost gone and
the rest of the tissue looks completely degenerated. What went wrong?
Heidi Röhrich
Histology Core Lab
Department of Ophthalmology
University of Minnesota
370 Lions research Building
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References
1. http://g.msn.com/8HMBENUS/2755??PS=47575
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