I recently bought a mouse monoclonal antibody against p-c-Jun from Santa Cruz Biotech (sc-822). I used this antibody for immunofluoresent staining of cryostat sections from injured rat spinal cord (4% PF perfusion of the rat). I got very strong non-specific labelling of the axons. Any kind advice of how to avoid such non-specific labelling? also anyone has a good protocol for this monoclonal antibody?

Many thanks,

Dr. WL Huang

Neuroscience Centre
Queen Mary University of London
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet