[Histonet] mouse monoclonal antibody for p-c-Jun
I recently bought a mouse monoclonal antibody against p-c-Jun from Santa Cruz Biotech (sc-822). I used this antibody for immunofluoresent staining of cryostat sections from injured rat spinal cord (4% PF perfusion of the rat). I got very strong non-specific labelling of the axons. Any kind advice of how to avoid such non-specific labelling? also anyone has a good protocol for this monoclonal antibody?
Dr. WL Huang
Queen Mary University of London
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