We have decided to try the Stable DAB from Phoenix and we would like to know what staining conditions others on histonet have used. The protocol calls for 5 + 5 minutes at 55C. Is this the condition that everyone is using or are people incubating at room temperature?
 
Please let me know as we are attempting to optimize a new protocol for an antibody we hope to use regularly.
 
Thanks,
 
Phil Huff
 
http://www.phxbio.com/

		
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