[Histonet] Re: Histonet Digest, Vol 9, Issue 27
Hello Dr. WL Huang,
In regards to the mouse monoclonal antibody for p-c-Jun:
Be sure to check if your secondary antibody is "rat adsorbed". I
suspect that you may be experiencing a cross reactivity due to the
species (rat/mouse) being so closely related. I have also done quite a
bit of immunoflourescent and immunoperoxidase staining of rodent neural
tissue from spinal cord injured rodents, and had this very same problem.
Also, I would suggest checking the Ig groups. For example, IgG versus
IgA. Did you use a protein blocking solution? If not you may want to
try that as well. Be sure to watch the concentration of your antibodies
and blocking serums/protein solutions. If the concentrations are too
high (say 1:250 [high] vs 1:1000[lower]) you will get a high
background. I suggest doing a titration method to see which
concentrations give th
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