[Histonet] RE: IHC polymer detection system for rat tissues
Just use the anti-mouse EnVision for use on rat tissue specimens. However, before using it you have to mix the EnVision with 10-15% (final concentration) normal rat serum. Leave the mixture for 60 min at room temp (or overnight 4C) then use it. All the cross-reactions are abolished now.
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center
Amsterdam - The Netherlands
----- Original Message -----
>From "Ronald P. Wilson"
Date Thu, 19 Aug 2004 15:15:43 -0400
Subject [Histonet] IHC polymer detection system for rat tissues
Has anyone found a successful polymer detection system (i.e., Envision or
others) directed against a mouse monoclonal on rat tissue. We tried one but
got a lot of background staining, I suspect from cross reactivity with
closely related antigens. Our standard IHC approach uses an anti-mouse,
rat-absorbed secondary antibody, but I would like to get the added
sensitivity of a polymer-based system.
Ronald P. Wilson, V.M.D., M.S.
Department of Comparative Medicine, HO54
Penn State University College of Medicine
M. S. Hershey Medical Center
500 University Drive
Hershey, PA 17033
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