[Histonet] RE: Histonet Digest, Vol 9, Issue 34

From:"Behan, Rosemarie G"

also seeking information--does anyone use "dry acetone"?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Saturday, August 21, 2004 1:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 9, Issue 34


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Today's Topics:

   1. RE: Spirit fixation of tissue (Pamela Marcum)
   2. Re: pancreas tissue (Richard Cartun)
   3. pancreas tissue (Fightmaster Janice)
   4. RE: doing immunos by hand (hymclab)
   5. RE: IHC polymer detection system for rat tissues
      (C.M. van der Loos)
   6. Hand IHC (madaryhtl@juno.com)
   7. Desperately seeking..information (Lesley S. Bechtold)
   8. Drying slides before Filing (Kopczynski, Charlotte)
   9. RE: Drying slides before Filing (Gary Gill)
  10. RE: Drying slides before Filing (Kevin Briggs)
  11. RE: Image analysis and slide scanning machine (Histo Jock)
  12. RE: Old IMLT journals (Edmondson David (RBV) NHS Christie Tr)


----------------------------------------------------------------------

Message: 1
Date: Fri, 20 Aug 2004 13:26:30 -0400
From: "Pamela Marcum" 
Subject: RE: [Histonet] Spirit fixation of tissue
To: "Edmondson David \(RBV\) NHS Christie Tr"
	, 	"'JOHN PHILLIPS'"
	
Cc: "Histonet \(E-mail 2\)" 
Message-ID: <000e01c486da$d4bdfd70$4f00a8c0@PMARCUM2K>
Content-Type: text/plain;	charset="iso-8859-1"

The Journals, the glass or the spirits, which of theses was the inheritance
here?  By the way a long time ago, in the 80's I remember a specimen being
sent (or at least it is what I was told) to the AFIP in tequila as fixative
and dehydrant.  We all mourned the lose of a good tequila as that is what
was used.  I think a cheap  one would have been just as good.

Pam Marcum


> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Edmondson
> David (RBV) NHS Christie Tr
> Sent: Friday, August 20, 2004 12:09 PM
> To: 'JOHN PHILLIPS'
> Cc: Histonet (E-mail 2)
> Subject: RE: [Histonet] Spirit fixation of tissue
>
>
> John,
> I have all the back issues of the IMLT/IMLS Journal so will have look, but
> slowly, over a glass.  ( I inherited them, honest )
> Dave
>
> -----Original Message-----
> From: JOHN PHILLIPS [mailto:JOHN.PHILLIPS@new-tr.wales.nhs.uk]
> Sent: 19 August 2004 14:33
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Spirit fixation of tissue
>
>
> Hello Histonet users,
>
> I would really appreciate knowing where to find an article,
> author unknown,
> published, probably somewhere in the time period 1972 to 1986,
> about the use
> of spirit for the fixation of skin biopsies and the like. I think it may
> have been published in the Institute of Medical Laboratory Technology
> journal (UK) or may be the Journal of Clinical Pathology (UK). The article
> investigated the effects of rum, whisky, gin and vodka etc. on tissue. I
> think rum came out on top.
>
> I am presenting a talk to GP's and would like to mention this as
> part of my
> presentation. I shall of course advise them not to use a Scottish
> malt under
> any circumstances that would be sacrilage. American bourbon may be!
>
> Thanks,
>
> John
>
> Mae'r e-bost hwn ac unrhyw ffeiliau a drosglwyddir gydag ef yn
> gyfrinachol ac wedi'u bwriadu ar gyfer pwy bynnag y cyfeirir ef ato neu
> atynt.  Os ydych wedi ei dderbyn drwy gamgymeriad yna gadewch i'r
> rheolwr systemau wybod drwy ddefnyddio'r manylion isod.
>
> Mae cynnwys yr e-bost hwn yn cynrychioli barn y sawl a enwir uchod,
> felly nid ydyw'n dilyn ei fod yn cynrychioli barn GIG Gogledd Ddwrain
> Cymru.
>
> Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i
> Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru roi cyhoeddusrwydd i gynnwys
> unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag
> amodau‚EUR(tm)r Ddeddf
> Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth,
> cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru
> ar  www.newalesnhstrust.org.uk
>
>
> This email and any files transmitted with it are confidential and
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------------------------------

Message: 2
Date: Fri, 20 Aug 2004 13:30:51 -0400
From: "Richard Cartun" 
Subject: Re: [Histonet] pancreas tissue
To: ,
	
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

I can.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> Fightmaster Janice  08/20/04
12:44PM >>>
I am in need of a block of normal pancreas tissue for IHC controls.
Can
anyone help me?

Janice Fightmaster
Pathology Supervisor
Oak Hill Hospital
11375 Cortez Blvd.
Brooksville, FL 34613
(352)597-6363 Ext. 3636


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------------------------------

Message: 3
Date: Fri, 20 Aug 2004 12:31:55 -0500
From: Fightmaster Janice 
Subject: [Histonet] pancreas tissue
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	<1734C20AE655CD449599A15EDC589D89017CA59D@orlex05.hca.corpad.net>
Content-Type: text/plain;	charset="iso-8859-1"

Thanks for the responses; I have plenty on the way now!! 

Janice Fightmaster
Pathology Supervisor
Oak Hill Hospital
11375 Cortez Blvd.
Brooksville, FL 34613
(352)597-6363 Ext. 3636




------------------------------

Message: 4
Date: Fri, 20 Aug 2004 13:30:45 -0500
From: hymclab 
Subject: RE: [Histonet] doing immunos by hand
To: "'Edmondson David (RBV) NHS Christie Tr'"
	,
"'Pathologyarts@aol.com'"
	
Cc: "Histonet \(E-mail 2\)" 
Message-ID:
	
Content-Type: text/plain

I have also used the Shandon sequenza for my manual immunos.  We have used
it for 10 years and love it!!!

Dawn Schneider, HT(ASCP)
Howard Young Medical Center
Woodruff, WI


-----Original Message-----
From: Edmondson David (RBV) NHS Christie Tr
[mailto:David.Edmondson@christie-tr.nwest.nhs.uk] 
Sent: Friday, August 20, 2004 11:29 AM
To: 'Pathologyarts@aol.com'
Cc: Histonet (E-mail 2)
Subject: RE: [Histonet] doing immunos by hand


Hi,
We use Shando's  "Sequenza" to do only a small number of slides these days
since a DAKO Techmate came along. The Sequenza has the option of an
organiser for several sets of slides but this is not necessary for small
numbers of slides The slides are arrayed each against plastic plates, in
groups of ten.  The plates forms a well at one end and solutions slowly
drain past, accross the face of the slides,  buffers antibodies, chromogens
, whatever.
In terms of a manual system it is sweeter than using trays, boxes.   Once
set in the clip there is no handling until the end.  The waste is held in
the container, staining is even , no ringing of the sections with a pen,
sweet.  Having done batches of a hundred with trays and then the sequenza,
my money is on the latter.

David Edmondson
Christie Hospital
Manchester UK


-----Original Message-----
From: Pathologyarts@aol.com [mailto:Pathologyarts@aol.com]
Sent: 19 August 2004 18:59
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] doing immunos by hand


does anyone still do immunos by hand or are they all done with these $40 
billion automated machines?

i would like to see what the options are as far as doing these things 
manually.

primarily for derm specimens, HMB 45, S 100, KI 67.


any help is appreciated,
curt
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------------------------------

Message: 5
Date: Fri, 20 Aug 2004 20:28:42 +0200
From: "C.M. van der Loos" 
Subject: [Histonet] RE: IHC polymer detection system for rat tissues
To: histonet@lists.utsouthwestern.edu
Cc: rpw4@psu.edu
Message-ID: 
Content-Type: text/plain; charset=us-ascii

Dear Ronald,
Just use the anti-mouse EnVision for use on rat tissue specimens. However,
before using it you have to mix the EnVision with 10-15% (final
concentration) normal rat serum. Leave the mixture for 60 min at room temp
(or overnight 4C) then use it. All the cross-reactions are abolished now.

Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center
Amsterdam - The Netherlands

----- Original Message ----- 
>From  "Ronald P. Wilson"  
Date  Thu, 19 Aug 2004 15:15:43 -0400 
To  Histonet  
Subject  [Histonet] IHC polymer detection system for rat tissues 
Has anyone found a successful polymer detection system (i.e., Envision or
others) directed against a mouse monoclonal on rat tissue. We tried one but
got a lot of background staining, I suspect from cross reactivity with
closely related antigens. Our standard IHC approach uses an anti-mouse,
rat-absorbed secondary antibody, but I would like to get the added
sensitivity of a polymer-based system.

Ronald P. Wilson, V.M.D., M.S.
Associate Professor
Department of Comparative Medicine, HO54
Penn State University College of Medicine
M. S. Hershey Medical Center
500 University Drive
Hershey, PA 17033
phone:  717-531-8460
fax:       717-531-5001
e-mail:  rpw4@psu.edu





------------------------------

Message: 6
Date: Fri, 20 Aug 2004 18:33:22 GMT
From: "madaryhtl@juno.com" 
Subject: [Histonet] Hand IHC
To: histonet@lists.utsouthwestern.edu
Message-ID: <20040820.113415.3885.451884@webmail23.lax.untd.com>
Content-Type: text/plain


40 Billion is alot to spend on a machine, I would shop around.  Sequenza
chambers serve us quite well as well as the automation.  All the stains you
mentioned are easily accomplished using hand techniqes.


Nick(Rocky) Madary, HT,HTL(ASCP)QIHC
Histology Manager, Medimmune Inc
Cell-3012334950
Work-3013984745
Fax-3013989745



------------------------------

Message: 7
Date: Fri, 20 Aug 2004 14:36:48 -0400
From: "Lesley S. Bechtold" 
Subject: [Histonet] Desperately seeking..information
To: histonet@Pathology.swmed.edu
Message-ID: <6.0.3.0.0.20040820142237.0261f600@aretha.jax.org>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Hi Everyone,

         Up here in Bar Harbor, Maine, we are trying to get an idea of whato
 ther Histology Labs are doing - how many people they have working in them,w
 hat they consider reasonable capacity, how much work they do in a year, 
how diversified they are, etc.  We are not interested in salary 
information.  We have created a bench-marking survey on our home website ath
 ttp://www.jax.org/sci_survey/ .  Click on the "Histology" link and it willt
 ake you right to the survey.  You'll notice there are other surveys on 
there as well so if your institution/hospital/business carries out any 
other activities (such as Electron Microscopy), please feel free to 
complete those surveys as well.  It only takes a few minutes to complete 
and will remain completely confidential.  Anyone who participates in the 
survey will receive a copy of the results (with names, etc. deleted) withina
  few weeks.  We're aiming for the beginning of October to send the resultst
 o all the participants.  We will need your email address in order to sendt
 he results to you so make sure you include that.

         So we'd really like to hear from you.  I know how active the 
Histonet is so I'm hoping for a good response as I'm sure everyone will bei
 nterested in the results.  For anyone who is not interested in 
participating, I apologize for using up your time.  Please delete this
email.

         Thank you!

Lesley Bechtold

Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191




------------------------------

Message: 8
Date: Fri, 20 Aug 2004 14:42:32 -0400
From: "Kopczynski, Charlotte" 
Subject: [Histonet] Drying slides before Filing
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	
<7C3A384EF3ABD611B3270008023E3E030481CC1C@mphexch01.mpm.baycare.internal>
	

Hello fellow Histonetters,

We are having a big problem with air bubbles under our coverslips before
filing.  We use Richard Allan mounting media and the slides in the trays are
placed in an oven set at 58 degrees C overnight.  I am thinking the oven may
be part of the problem but was wondering what the process is out there in
Histo-land.  

Thanks,
Charlotte Kopczynski,HTL(ASCP)
Baycare Pathology Manager
Phone: 727-461-8246
Fax:   727-462-7597

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Message: 9
Date: Fri, 20 Aug 2004 13:54:08 -0500
From: Gary Gill 
Subject: RE: [Histonet] Drying slides before Filing
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID: <0B11620AEE5EFB4FA7A7558339972A830704F3@HALIBUT.dcla.com>
Content-Type: text/plain

Air bubbles form because there is not enough solid material remaining under
the cover glass after the solvent has evaporate.  Among possible causes:

*	RAS put out a bad batch of mounting medium (i.e., more solvent than
usual)
*	Not enough mounting medium being applied
*	Accelerated drying (e.g., oven too hot [58 degrees C is not too hot,
in my experience]) -- how soon after coverslipping are slides put in oven?
Right away could make a negative difference.

Simplest first solution is to add more mounting medium, while appreciating
that way too much degrades image quality under 40x objectives -- which would
be the subject of another discussion.

Little useful information has ever been published about mounting media.  The
best information was published as a 2-part article in Stain Technology in
the 1950s.  Authors were a committee chaired by the late and great Dr. Ralph
D. Lillie.

Gary Gill

-----Original Message-----
From: Kopczynski, Charlotte [mailto:Charlotte.Kopczynski@baycare.org] 
Sent: Friday, August 20, 2004 1:43 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Drying slides before Filing


Hello fellow Histonetters,

We are having a big problem with air bubbles under our coverslips before
filing.  We use Richard Allan mounting media and the slides in the trays are
placed in an oven set at 58 degrees C overnight.  I am thinking the oven may
be part of the problem but was wondering what the process is out there in
Histo-land.  

Thanks,
Charlotte Kopczynski,HTL(ASCP)
Baycare Pathology Manager
Phone: 727-461-8246
Fax:   727-462-7597

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error please notify the system manager or  the 
sender immediately and do not disclose the contents to anyone or make
copies.

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------------------------------

Message: 10
Date: Fri, 20 Aug 2004 15:21:37 -0400
From: Kevin Briggs 
Subject: RE: [Histonet] Drying slides before Filing
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	<8A37FE7D529ED411983E00D0B75D01FC0663A79C@sirius.drmc.drhsi.org>
Content-Type: text/plain;	charset="iso-8859-1"

Charlotte,

	I think Gary's got it.  58 degrees C should only be too hot if the
slides were placed in the oven too soon after mounting as we have
found--although, decreasing the temperature will indeed slow the solvent's
rate of evaporation.  Try delaying your oven cure time and see if that
helps. 

Good luck!

Kevin D. Briggs, MS,CT(ASCP)
Team Leader- Cytopathology/Histopathology Services
Danville Regional Medical Center
142 South Main Street
Danville, VA 24541
Telephone: (434) 799-4470 ext.5451
Fax: (434) 799-2118
E-mail: briggsk@drhsi.org


-----Original Message-----
From: Gary Gill [mailto:garygill@dcla.com]
Sent: Friday, August 20, 2004 2:54 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Drying slides before Filing


Air bubbles form because there is not enough solid material remaining under
the cover glass after the solvent has evaporate.  Among possible causes:

*	RAS put out a bad batch of mounting medium (i.e., more solvent than
usual)
*	Not enough mounting medium being applied
*	Accelerated drying (e.g., oven too hot [58 degrees C is not too hot,
in my experience]) -- how soon after coverslipping are slides put in oven?
Right away could make a negative difference.

Simplest first solution is to add more mounting medium, while appreciating
that way too much degrades image quality under 40x objectives -- which would
be the subject of another discussion.

Little useful information has ever been published about mounting media.  The
best information was published as a 2-part article in Stain Technology in
the 1950s.  Authors were a committee chaired by the late and great Dr. Ralph
D. Lillie.

Gary Gill

-----Original Message-----
From: Kopczynski, Charlotte [mailto:Charlotte.Kopczynski@baycare.org] 
Sent: Friday, August 20, 2004 1:43 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Drying slides before Filing


Hello fellow Histonetters,

We are having a big problem with air bubbles under our coverslips before
filing.  We use Richard Allan mounting media and the slides in the trays are
placed in an oven set at 58 degrees C overnight.  I am thinking the oven may
be part of the problem but was wondering what the process is out there in
Histo-land.  

Thanks,
Charlotte Kopczynski,HTL(ASCP)
Baycare Pathology Manager
Phone: 727-461-8246
Fax:   727-462-7597

****************************************************************************
**********************
The contents of this email and any attachments are confidential. They are
intended for the named recipient(s) only. If you have received this email in
error please notify the system manager or  the 
sender immediately and do not disclose the contents to anyone or make
copies.

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****************************************************************************
**********************

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------------------------------

Message: 11
Date: Fri, 20 Aug 2004 22:26:42 -0400
From: "Histo Jock" 
Subject: RE: [Histonet] Image analysis and slide scanning machine
To: histonet@lists.utsouthwestern.edu
Cc: gsmith@confocal.com
Message-ID: 
Content-Type: text/plain; format=flowed


Can you describe more about how this works? How does it create the image ifi
 t doesn't tile? What kind of analysis can it do? You're exhibiting with 
Beecher - can it run Beecher's new software?

HistoJock.

>Message: 9
>Date: Mon, 16 Aug 2004 16:11:45 -0400
>From: "Glenn Smith" 
>Subject: [Histonet] Image analysis and slide scanning machine (Wester,
>	Martha)
>To: 
>Message-ID: <00c101c483cd$45edc040$1f05a8c0@confocal.com>
>Content-Type: text/plain;	charset="US-ASCII"
>
>Hi Kim,
>
>We supply such an instrument, our TISSUEscope - combining confocal scanning
>laser microscopy and a wide field of view.  Depending on your resolution
>requirements, the TISSUEscope can acquire a single image at up to 1 um
>resolution with no mosaic and no tiling of images. A standard slide (1" x
>3") is loaded into the machine and the rest of operations is via software.
>We have another model which offers submicron resolution as well.  Both
>brightfield and fluorescence detection is provided within the same
>instrument.  Please feel free to contact me offline for more details.
>
>Also, we will have one of these instruments at the NSH meeting in Toronto -
>we'll be exhibiting in conjunction with Beecher Instruments.
>
>Regards
>Glenn Smith, P.Eng.
>519.886.9013 x38
>
>Biomedical Photometrics Inc/GeneFocus www.GeneFocus.com>
>Widefield Confocal Scanning Instruments and Software

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------------------------------

Message: 12
Date: Sat, 21 Aug 2004 11:14:12 +0100
From: "Edmondson David (RBV) NHS Christie Tr"
	
Subject: [Histonet] RE: Old IMLT journals
To: 'Bryan Llewellyn' 
Cc: "Histonet \(E-mail 2\)" 
Message-ID:
	

	
Content-Type: text/plain;	charset="iso-8859-1"

OK Bryan,
It will take a little while, but I shall start.  I wonder that someone has
already scanned some journals, like "path and bact", stain tech and
histochem journal.  Or at least found the annual indexes to scan.  There are
Stain tech 69-83 and Histochen J 79-86 beside me now, and I wonder at the
quantity of published material.   
Maybe I shall ask the IBMS,  I remember there is/was a historical section
that may have moved from preservation to digitising.   Not the same as
"Brass and Glass", but.  


Dave

-----Original Message-----
From: Bryan Llewellyn [mailto:bryand@netbistro.com]
Sent: 20 August 2004 20:41
To: Edmondson David (RBV) NHS Christie Tr
Subject: Old IMLT journals


Hi,
I have a big favour to ask.  I am the author of the StainsFile web site 
(http://stainsfile.info).  One of my aims for this site is to include 
all published staining methods.  I know that's quite futile, but I would 
like to get as many as possible.

I have the IMLT journals between 1962 and about 1975, when I let my 
membership finally lapse after emigrating to Canada.  Is there any 
possibility that I could get you to scan or photocopy any histological 
methods involving techniques or theory about dyes or metallic 
impregnations (almost everything except immuno- or enzyme histochemistry 
as these are outside my limits for the site) from any journals you have 
apart from those years?  I would like to be able to include the material 
on StainsFile, and I have been wondering how I might be able to get 
details from Canada.   Unfortunately, none of the British Techs I know 
over here has kept their copies, or have let their membership lapse.

Here's hoping.

Bryan Llewellyn


Edmondson David (RBV) NHS Christie Tr wrote:
> John,  
> I have all the back issues of the IMLT/IMLS Journal so will have look, but
> slowly, over a glass.  ( I inherited them, honest )
> Dave
> 
> -----Original Message-----
> From: JOHN PHILLIPS [mailto:JOHN.PHILLIPS@new-tr.wales.nhs.uk]
> Sent: 19 August 2004 14:33
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Spirit fixation of tissue
> 



------------------------------

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