[Histonet] IHC polymer detection system for rat tissues
Has anyone found a successful polymer detection system (i.e., Envision or
others) directed against a mouse monoclonal on rat tissue. We tried one but
got a lot of background staining, I suspect from cross reactivity with
closely related antigens. Our standard IHC approach uses an anti-mouse,
rat-absorbed secondary antibody, but I would like to get the added
sensitivity of a polymer-based system.
Ronald P. Wilson, V.M.D., M.S.
Department of Comparative Medicine, HO54
Penn State University College of Medicine
M. S. Hershey Medical Center
500 University Drive
Hershey, PA 17033
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