[Histonet] High pH AR
|From:||"Cesar Francisco Romero" |
I have had terrible results with Tris-Clorhidric.
I prefer de following buffer:
Tris/EDTA pH 9 (10/1 mM)
Buffer may be produced as follows:
1,21 g Tris, Merck 1.08387
0,37 g EDTA, Fluka Chemika 03680
1000 ml destilled water
Buffer pH is stable at 9.0 - 9.1, usually without adjustment. The buffer is
stable for approximately 4 weeks at room temperature. I keep it in the
icebox for several weeks. I add two drops per 1000 ml of tween 20.
MSN Amor: busca tu ½ naranja http://latam.msn.com/amor/
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