[Histonet] H&E/Alcian Blue
|From:||Jose Luis Palazon Fernandez |
I would like to perform an H&E/Alcian blue staining to differentiate bone and cartilage and I was wondering if I have to do the H&E first and then AB or the contrary or maybe there is a protocole to this staining technique. The acid AB solution will bleach the Hematoxylin?. If the AB is used first why not call the technique AB/H&E instead of H&E/AB?
thanks in advance
Universidad de Oriente-Isla Margarita-Venezuela
actualmente en: Instituto de Ciencias Marinas de Andalucia
Puerto Real, Cádiz, España.
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