[Histonet] Golgi Cox staining


I try to establish a Golgi Cox staining for mouse brain tissue. After the
first trials, I have two major problems:

1) The slices (between 20 and 80µm) cut from paraffin blocks using a
sliding microtome are rather fragile.

2) There are so many neurones stained, that I do not know, how to
distinguish between them and how to quantify the data.

Does anybody have an idea to solve these problems?

Thank you, and kind regards

Oliver Ambrée


Oliver Ambrée

Institute of Neuropathology

University Hospital Münster

Domagkstr. 19

D - 48149 Münster

phone: +49 251/ 83-52359

fax: +49 251/ 83-56971



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