I try to establish a Golgi Cox staining for mouse brain tissue. After the
first trials, I have two major problems:

1) The slices (between 20 and 80µm) cut from paraffin blocks using a
sliding microtome are rather fragile.

2) There are so many neurones stained, that I do not know, how to
distinguish between them and how to quantify the data.

Does anybody have an idea to solve these problems?



Thank you, and kind regards



Oliver Ambrée





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Oliver Ambrée

Institute of Neuropathology

University Hospital Münster

Domagkstr. 19

D - 48149 Münster



phone: +49 251/ 83-52359

fax: +49 251/ 83-56971

ambree@uni-muenster.de

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