[Histonet] Caspase 3 on mouse spleen
Good Morning Histonetters -
Does anyone out there have experience with caspase-3 on mouse spleen? I
routinely perform Caspase-3 staining on xenografts, some gut, and a liver
now and then - but just started looking at some spleens. They're a mess.
I see what appears to be a lot of nonspecific staining of histiocytes in
the red pulp. I'm staining immunocompetent mice, and repeated the stain
with a naive SCID spleen - same staining pattern. Can anyone give me any
insight? I'm using Pharmingen's 55965, Rabbit anti active Caspase-3. I've
tried DAKO Envision, as well as an SABC method and get exactly the same
staining. I'm a bit baffled. Any tidbits?
Jacqueline M. O'Connor HT(ASCP)
Global Pharmaceutical Research and Development
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