[Histonet] Caspace-3 on rat tissue

From:"Ronald P. Wilson"

In response to a question about Caspace-3 for mouse spleen, Liz Chlipala
mentions using this antibody on rat, mouse and human tissue. My question to
Liz and others, anyone have experience with this antibody and rat skin. We
tried it a few weeks ago using rat intestine and skin and pH 6.0 citrate
buffer HIER. We did get positive staining on the intestine tissue but not on
the skin. The skin is our tissue of interest. It was suggested to us by the
manufacturer that perhaps Caspace-3 is not expressed or has very low
expression in the skin. Anyone know the answer. I would love to hear if
others have any experience with rat skin.

Ronald P. Wilson, V.M.D., M.S.
Associate Professor
Department of Comparative Medicine, HO54
Penn State University College of Medicine
M. S. Hershey Medical Center
500 University Drive
Hershey, PA 17033
phone:  717-531-8460
fax:       717-531-5001
e-mail:  rpw4@psu.edu

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