FW: [Histonet] pHing of eosin

From:"Barry R Rittman"

I believe that care should  be used in universally adding  specific amounts of acetic acid to acidify eosin solutions that differ. 

	I asume that different batches of dye have different dye contents so that the 5% stain that one user prepares is a little different from that used by others. Each lab should prepare their eosin with this is mind.
	I usually add a low percentage of acetic acid to the eosin until a faint opalescence occurs. I have been told that this is the point at which the dye acid just starts to precipitate. 
	For our eosin solutions this involves 5  drops or so of 2% acetic acid per 100 ml of 5% eosin.

		-----Original Message----- 
		From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tony Henwood 
		Sent: Sun 8/15/2004 6:27 PM 
		To: 'Daryl Mikita'; histonet@lists.utsouthwestern.edu 
		Subject: RE: [Histonet] pHing of eosin

		We use 3% acetic acid
		Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
		Laboratory Manager
		The Children's Hospital at Westmead,
		Locked Bag 4001, Westmead, 2145, AUSTRALIA.
		Tel: 612 9845 3306
		Fax: 612 9845 3318
		-----Original Message-----
		From: Daryl Mikita [mailto:dmikita@wmcnet.org]
		Sent: Saturday, 14 August 2004 5:44 AM
		To: histonet@lists.utsouthwestern.edu
		Subject: [Histonet] pHing of eosin
		We are looking at pHing our eosin to the correct pH, instead of just using
		it straight from the bottle.  What should we use to adjust the pH?  Should
		it be 1N sodium hydroxide or  1N hydrochloric acid? 
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