FW: [Histonet] doing immunos by hand

From:Patti Loykasek

------ Forwarded Message
From: Patti Loykasek 
Date: Fri, 20 Aug 2004 09:35:03 -0700
To: Hermina Borgerink 
Subject: Re: [Histonet] doing immunos by hand

Curt, there are a couple of ways to go about it. Several involve little
expense.  The way we do it most frequently is to use either micro culture
trays that have a lid or cafeteria trays. Either way, you will need to use
some bench top paper that is a foam type material on one side & absorbent
type material on the other. Cut the paper to fit your tray. Wet down the
paper side with a water squirt bottle & smooth out the paper to eliminate
bubbles by rolling a small tube or pipette over the surface. If using the
micro trays, lay the paper inside the tray, lay your slides in the tray,
cover with lid for incubation. These micro trays hold about 20-22 slides.
For more slides, lay the bench paper on the counter top, wet & smooth. Lay
your slides on top, cover with the cafeteria tray to create a "lid" for your
incubation chamber. If you need more info, please contact me. Hope this
helps  & gives you some ideas.
For ease of handling, you may want to investigate the MicroProbe from
Fisher. It operates on the capillary gap principle. You must use cap. Gap
slides. It holds 10 pairs of slides. Not very expensive. Has heat, can do
in-situs, too.

Patti Loykasek
PhenoPath Laboratories
Seattle, WA  

> I do all my immunos by hand (up to 80 slides at a time) using the
> MicroProbe Staining system from Fisher.  The system operates by means of
> special slides that have a painted surface and a special slide holder.
> When paired together (facing each other) a capillary gap is created that
> allows fluids to rise between the two slides using capillary action.
> You don't need to purchase the complete set up either, just the special
> slide holders and isolons (rubber pads with wells to dispense reagents).
> I think each slide holder (to stain 20 slides at once) runs around
> $250.00.  I'm not sure about the isolons, but they are not that expense
> and are re-usable. I routinely use a large panel of antibodies,
> including the ones you mentioned. Check the Fisher catalog for more
> details.
> Hermina 
> Hermina M. Borgerink, BA, HTL(ASCP)QIHC
> Wake Forest University Health Sciences
> Department of Pathology
> Medical Center Blvd.
> Winston-Salem, NC 27157
> Tel. (336) 716-1538
> Fax (336) 716-1515
> e-mail hborgeri@wfubmc.edu
> "Treat a man as he appears to be, and you make him worse.  But treat a
> man as if he already were what he potentially could be, and you make him
> what he should be."  (Goethe)
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> Pathologyarts@aol.com
> Sent: Thursday, August 19, 2004 1:59 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] doing immunos by hand
> does anyone still do immunos by hand or are they all done with these $40
> billion automated machines?
> i would like to see what the options are as far as doing these things
> manually.
> primarily for derm specimens, HMB 45, S 100, KI 67.
> any help is appreciated,
> curt
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