Re: [Histonet] Staining for Macrophages
Thanks, Gail, you're right. Does FA-11 work on FFPE tissue and do
you need HIER? Serotec doesn't say.
>Sorry, but ED-1 (CD68) is a Mouse anti-Rat antibody for a subset of
>rat macrophages. It may cross react to mouse but you would be doing
>doing mouse on mouse IHC. The CD68 for mouse is rat anti-mouse,
>clone FA-11 for murine peritoneal macrophages. SEROTEC is a source
>of these antibodies, including the F4/80 (Rat anti-mouse).
>A sidenote: SEROTEC has a free macrophage differentiation poster
>available upon request.
>At 06:58 PM 8/4/2004, you wrote:
>>You can use ED-1 from Serotec. It works on FFPE tissue with pH 6.0
>>citrate antigen retrieval and on frozen tissue without AR. You can
>>also use F4/80 on frozen tissue. Both are rat monoclonals and you
>>can use an anti-rat secondary Ab cross adsorbed against mouse IgG.
>>You could also also use ricin 1 from Vector or E-Y Laboratories
>>which binds mouse macrophages. Ricin 1 is not the dangerous ricin.
>>I don't know details of how to use it (I did once, but I don't
>>remember). There are books available or the manufacturers may know
>>or maybe some other Histonet folks. I have used ED1 and F4/80 on
>>mouse tissues and I think it's your best bet.
>>>I had previously asked about MAC-387 staining. Unfortunately, we
>>>can't use the staining because we are staining mouse kidney and it
>>>doesn't look right if you stain with a mouse anti-body. Does
>>>anyone know of a way to stain for macrophages with out an
>>>antibody??? Or perhaps a polyclonal antibody???? Thanks a bunch!
>>>Jennifer K. Sipes, RALAT
>>>Sr. Laboratory Technician
>>>Johns Hopkins University
>>>720 Rutland Avenue
>>>Baltimore, MD 21205
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