Re: [Histonet] Staining for Macrophages

From:Sharon Cooperman

You can use ED-1 from Serotec.  It works on FFPE tissue with pH 6.0 
citrate antigen retrieval and on frozen tissue without AR.  You can 
also use F4/80 on frozen tissue.  Both are rat monoclonals and you 
can use an anti-rat secondary Ab cross adsorbed against mouse IgG. 
You could also also use ricin 1 from Vector or E-Y Laboratories which 
binds mouse macrophages.  Ricin 1 is not the dangerous ricin.  I 
don't know details of how to use it (I did once, but I don't 
remember).  There are books available or the manufacturers may know 
or maybe some other Histonet folks.  I have used ED1 and F4/80 on 
mouse tissues and I think it's your best bet.


>I had previously asked about MAC-387 staining.  Unfortunately, we 
>can't use the staining because we are staining mouse kidney and it 
>doesn't look right if you stain with a mouse anti-body.  Does anyone 
>know of a way to stain for macrophages with out an antibody???  Or 
>perhaps a polyclonal antibody????  Thanks a bunch!
>Jennifer K. Sipes, RALAT
>Sr. Laboratory Technician
>Johns Hopkins University
>Ross 929
>720 Rutland Avenue
>Baltimore, MD  21205
>phone:  410-614-0131
>cell:     443-413-0853
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Sharon Cooperman       	     
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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