Re: [Histonet] Staining for Macrophages

From:Gayle Callis

There are other monoclonal antibodies for macrophages in the mouse, 
generally rat antimouse, try BD Pharmingen.  F4/80 (mature macrophages) is 
another available from Serotec.  You can use a mouse monoclonal on mouse 
tissue, but should use the DAkO ARK kit or Chemicon mouse on mouse kit, 
there are others on the market.  Hopefully, you have a positive control 
expressing the macrophage population you wish to see, this way you can 
optimize your antibody before going to experimental animal.

  At 08:28 AM 8/4/2004, you wrote:
>I had previously asked about MAC-387 staining.  Unfortunately, we can't 
>use the staining because we are staining mouse kidney and it doesn't look 
>right if you stain with a mouse anti-body.  Does anyone know of a way to 
>stain for macrophages with out an antibody???  Or perhaps a polyclonal 
>antibody????  Thanks a bunch!
>Jennifer K. Sipes, RALAT
>Sr. Laboratory Technician
>Johns Hopkins University
>Ross 929
>720 Rutland Avenue
>Baltimore, MD  21205
>phone:  410-614-0131
>cell:     443-413-0853
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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