Re: [Histonet] Fluorescence and photobleaching discussion

From:Gayle Callis

Teri et al,

The same bubble problem/media retraction problem occurs with Molecular 
Probes Prolong Gold ready to use and that is why they suggest sealing edges 
of coverslip for long term storage.  Prolong Gold is thicker in consistency 
but be sure to seal down edges.  I have this occur with AquaMount, an all 
purpose aqueous mounting media with AEC chromogen - edges get sealed with 
this media too.

Gayle Callis

At 03:17 PM 8/12/2004, you wrote:
>Gayle writes: **Vector has VectaShield Hard Set that is supposed to work
>also.  It is
>nice to have an aqueous mounting media for fluorescent work that sets up
>I have used this product and I don't like it.  Initially I did because
>it is thin in consistency and spreads well. However, with time, thick
>specimens (12 microns) developed air pockets in some of them.  Having
>learned this, I used more on the next batch of slides, and ended up with
>the same problem.  Also, they state that you can easily remount if
>necessary by soaking in water to remove the coverslip.  It took 3 days
>before the coverslip was loose enough to remove, and remounting wasn't
>really satisfactory for the coverslips that had cultured cells on it.
>Some of the cells adhered to the glass slide.
>Others in our facility use this stuff and love it.
>Teri Johnson
>Managing Director Histology Facility
>Stowers Institute for Medical Research
>1000 E. 50th St.
>Kansas City, Missouri  64110
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