Re: [Histonet] F4/80 antibody

From:Gayle Callis

Histonet archives (www.histosearch.org) has informtation on F4/80 (you 
should do a dilution panel on tissue expressing mature macrophages to 
determine optimal working concentration adn start at 10 ug/ml.)

 From one Histonet message, Barb Wright did Pretreatment:  0.4% Pepsin in 
0.1N HCl (5 minutes at 37C).    As for secondary, we use TAGO/Biosource=20
goat antirat F(ab')2 frag of IgG, biotinylated adsorbed to mouse or Jackson=20
Donkey antiRat F(ab')2 frag of IgG-biotinylated adsorbed to mouse (and 
other species) 0.5 mg/ml dilutrd 1:250.  If you do not have success with 
the ABCAM antibody, buy SEROTEC - it works very well.





Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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