Suggest using glacial acetic acid: 5 mL per liter of eosin.  Be prepared for
a color explosion.

Gary Gill

-----Original Message-----
From: Daryl Mikita [mailto:dmikita@wmcnet.org] 
Sent: Friday, August 13, 2004 2:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] pHing of eosin


Hello,

We are looking at pHing our eosin to the correct pH, instead of just using
it straight from the bottle.  What should we use to adjust the pH?  Should
it be 1N sodium hydroxide or  1N hydrochloric acid?  

Thanks
Daryl


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