Dana,

There was heaps written about this on Histonet previously, look in the
archives.
We found that the problem was associated with the Plus slides when used in
conjunction with an EDTA buffer as the material used to coat the slides is a
calcium salt and the EDTA was affecting the coating by chelating the
calcium!!!

All the best
Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
DDittus787@aol.com
Sent: Friday, 06 August 2004 12:10 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] electrical charge slides


To my colleagues:

I have a question.Is anyone out there having occasional staining(orlack of
staining on IHc slides?) I was told that the charge or the way the slides
are charged can have (pardon the pun) a negative effect. Does anyone know
/experience or have the scientific data for this?
                                 Dana

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