We use 20% nitric acid in distilled water.  We let it sit in the glassware for at least 30 minutes then pour it back into the container (we re-use the solution for 3 months) and then thoroughly rinse the glassware with distilled water.  We clean the glassware just before use and we primarily use acid cleaned glassware for silver procedures. We routinely have our glassware cleaned by our glassware department which uses strong detergents and high temperatures so the glassware is cleaned very well but we still acid clean for our silver procedures.
 
Jeanine Bartlett
CDC

	-----Original Message----- 
	From: histonet-bounces@lists.utsouthwestern.edu on behalf of Diana McCaig 
	Sent: Fri 8/13/2004 6:03 PM 
	To: histonet@lists.utsouthwestern.edu 
	Cc: 
	Subject: [Histonet] acid washed glassware
	
	

	What concentration  and what acid do you use to clean glassware prior to
	silver impregnation staining.  Do you simply rinse it or is it soaked for a
	specific period of time?  Air or oven dried?
	Diana McCaig, R.T.
	Charge Tech, Histology
	Chatham Kent Health Alliance
	519-352-6401 (6604)
	
	
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