RE: [Histonet] Staining for Macrophages

From:"Dardo Ferrara"

mac-3 rat antimouse(BD pharmingen) at 1:500-1:200 works perfectly in IHC 
samples.



>From: Jennifer Sipes 
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] Staining for Macrophages
>Date: Wed, 4 Aug 2004 07:28:56 -0700 (PDT)
>
>I had previously asked about MAC-387 staining.  Unfortunately, we can't use 
>the staining because we are staining mouse kidney and it doesn't look right 
>if you stain with a mouse anti-body.  Does anyone know of a way to stain 
>for macrophages with out an antibody???  Or perhaps a polyclonal 
>antibody????  Thanks a bunch!
>
>
>Jennifer K. Sipes, RALAT
>Sr. Laboratory Technician
>Johns Hopkins University
>Ross 929
>720 Rutland Avenue
>Baltimore, MD  21205
>phone:  410-614-0131
>cell:     443-413-0853
>e-mail:  jengirl1014@yahoo.com
>
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