RE: [Histonet] Spleen autofluorescence

From:"Bill Sinai"

Many many years ago I had a similar problem in gut sections.  The
autofluorescence after formaldehyde fixation was very strong in the
argentaffin cells.  Our Histochemist at that time suggested I do a
haematoxylin stain (very briefly Mayer's 30sec - 1min no differentiation)
and I was very surprised when it quenched most of the autofluoresence.

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Ph 02 9845 7774

-----Original Message-----
[]On Behalf Of Sharon
Sent: Thursday, 05 August 2004 6:03 AM
To: Helen Newbery
Subject: Re: [Histonet] Spleen autofluorescence

I have had pretty good luck quenching autofluorescence in FFPE spleen
with sodium borohydride 0.5 mg/ml in PBS pH 8.0 x 10 minutes x 3
changes.  It works better than H2O2 for me.


>Hi histonetters,
>Apologies if my query is really basic but I am wanting to do
>antibody staining on mouse spleen sections (paraffin-embedded) and am
seeing a
>lot of autofluorescence, which I presume is due to platelets. Is there any
>around this problem?
>Helen Newbery,
>Medical Genetics Section,
>Molecular Medicine Centre,
>Western General Hospital,
>EH4 2XU.
>0131 651 1047
>Histonet mailing list

Sharon Cooperman       	     
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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