RE: [Histonet] RE: replies to immunostaining on technovit 8100

From:"Webster, Paul"

There is a new resin that has just bee released called Technovit 9100 New. I have no experience with this resin but in addition to polymerizing at low temperatures, it is also possible to remove the resin from tissue sections using 2-methoxyethyl acetate. 

There is a paper describing its use in European Cells and Materials 2003 vol 6 pages 57-71. Authors are Yang, R., CM Davies, CW Archer and RG Richards.

Any comments from people who may have used this resin would be of interest to me.


Paul Webster.

Paul Webster, Ph.D.
House Ear Institute
2100 West Third Street
Los Angeles
CA 90057
phone (213) 273 8026
fax      (213) 413 6739

> ----------
> From: on behalf of PALMER Jason (SVHM)
> Sent: 	Thursday, August 12, 2004 7:13 PM
> To:
> Subject: 	[Histonet] RE: replies to immunostaining on technovit 8100
> <><>
> 	Thanks Pam Marcum and Gayle (especially) for your thoughtful responses.
> 	Perhaps I should have been clearer as to why we want to use this rather than paraffin - the tissues we collect are grown on a PLGA (a polymer of lactic and glycolic acids) scaffold, which melts / distorts at any temp above 45 degrees c, not to mention in alcohols,acetone, xylene and the like. Hence our desire to avoid paraffin. Technovit 8100 polymerises at zero degrees, so no heat needed at all, and we use inert dehydration to remove excess water from the PLGA / tissues. And, supposedly immunohistochemistry compatible....
> 	And yes, I got the mouse and rat confused with the antibody - mouse anti rat ED1 it is!
> 	Cheers,
> 	Jason Palmer
> 	Bernard O'Brien Institute of Microsurgery
> 	Melbourne, Australia

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