We use an immunohistochemical antibody to cytokeratin 7.  Chromophobe
carcinomas are positive for CK7, while oncocytomas are negative (or
minimally positive). [Leroy et al, "Utility of cytokeratin 7 for
distinguishing chromophobe renal cell carcinoma from renal oncocytoma."  Eur
Urol 2000; 37: 484-487, also in Dabbs immunohistochemistry text, pp
477-481.] We've found it a lot easier to do and interpret than colloidal
iron.  We get our antibody from Biocare (clone K72.7), and run it on a
Ventana immunostainer with protease predigestion, but I think a lot of
immunohistochemistry companies carry cytokeratin 7 antibodies, so you could
check your catalogs and see what's out there.

Gloria Niehans
Minneapolis VAMC


-----Original Message-----
From: Barnhart, Tammy [mailto:Tbarnhart@primecare.org]
Sent: Friday, August 06, 2004 6:11 AM
To: Histonet (E-mail)
Subject: [Histonet] Oncocytoma vs. Chromophobe


I was wondering if there is(are) any immunohistochemical antibodies or
special stains besides Hale's Colloidal Iron for the differentiation of
Oncocytoma and Chromophobe in kidney?  I am not able to make the dialysised
(sp?) iron needed for the Hale's but would like to offer my pathologist an
alternate.  Any suggestions?

Tammy Barnhart, BS, HTL(ASCP)
Anatomic Pathology Supervisor
St. Alexius Medical Center
Bismarck, ND




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