RE: [Histonet] Glass vs. Tape Coverslippers

From:"Weems, Joyce"

I was demoing coverslippers and didn't tell the pathologists. Thought I'd see if they could tell the difference. One of our pathologists made us remove the tape from her entire days workload and recoverslip them by hand. Oh well..... Joyce-----Original Message-----From:[]On Behalf Of Morken,Tim - LabvisionSent: Thursday, August 05, 2004 12:08 PMTo: 'Histonet (E-mail)'Subject: RE: [Histonet] Glass vs. Tape CoverslippersGary, We did find that plastic was no good for cytology specimens - at leastthe lumpy ones - it wouldn't form a flat surface. For thin-prep it workedgreat. I think the quality is OK for routine histology. In our lab we didtesting in the lab and when satisfied it was working well we switched overwithout telling the pathologists. About two weeks later the ChiefPathologist asked me when we would start using the plastic coverslips!Tim Morken-----Original Message-----From: Gary Gill [] Sent: Thursday, August 05, 2004 8:53 AMTo: 'Laurie Colbert';; Histonet (E-mail)Cc: histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] Glass vs. Tape CoverslippersPlastic is no substitute for glass.  Just because one doesn't "see" adifference doesn't mean there isn't a difference.  Whether the differencemakes a real difference in outcomes is another story.ASTM specs for cover glasses apply to glass, not to tape.  The higher thenumerical aperture of an objective, and the better the objective quality(i.e., achromat, fluorite, apochromat -- plan and non-plan), the more likelythat one will see imaging differences when tape is used.  Of course, usingglass doesn't ensure good quality.Practical stuff like mounting medium and cover glass thickness, cleanlenses, and Kohler illumination also play a real role.  Image quality can'tbe better than the weakest link.The specs are relative to the impact of the physical and optical propertiesof glass on light as it passes through the mounting medium and glass throughthe objective.  For this reason, it's not nice to fool Mother Nature and useplastic.Gary Gill-----Original Message-----From: Laurie Colbert [] Sent: Thursday, August 05, 2004 10:23 AMTo:; Histonet (E-mail)Cc: histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] Glass vs. Tape CoverslippersWe have a tape coverslipper, and we love it.  It is fast and we have hadvery few problems with it.  Our pathologists have no problem reading theslides, and as far as I know, there's never been a problem photographing aslide.  We did demo the glass coverslippers when we were first looking for anew coverslipper, and there were too many problems with slides stickingtogether, air bubbles, and it was just all-around not as "user friendly."Laurie ColbertHuntington Hospital-----Original Message-----From: []Sent: Wednesday, August 04, 2004 8:05 PMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Glass vs. Tape CoverslippersHello again, I'm looking for opinions on the subject of glass coverslippers versus tape  coverslipping.  I have the opportunity to decide on a system.  My only  experience has been with tape coverslipping.  I understand machines  thatglass coverslip are slower than tape systems. Is the refractive index  better withglass coverslips under the microscope?  Opinions pros/cons are  appreciated. Deb King, HT(ASCP)Sacramento, CA_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu mailing listHistonet@lists.utsouthwestern.edu mailing listHistonet@lists.utsouthwestern.edu mailing listHistonet@lists.utsouthwestern.edu Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer.Thank you. Saint Josephs Health System, Inc.

<< Previous Message | Next Message >>