I just recently finished a research project staining for macrophages in
mouse lungs.  I used an F4/80 Rat anti-mouse from Serotec

I used positive charged slides and enzyme pretreatment and did not have any
trouble with tissue washing off, but lungs can be more forgiving than skin.

I used the Pro-K from Dako for pretreatment, Primary was diluted 1:50 fro
60min, and the Vector Elite Rat setup.

Lawrence J Faucette

Contractor

HT ASCP

 
http://www.niaid.nih.gov/dir/services/animalcare/VetPathology/VetPathology-i
ndex.html

Infectious Disease Pathogenesis Section

Comparative Medicine Branch 

Division of Intramural Research, NIAID, NIH

Twinbrook III, Room 2W-01A, MSC 8135

12735 Twinbrook Parkway

Bethesda, MD 20892-8135

Telephone 301-451-1056

Fax 301-480-2343

SoBran, Inc.

 

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-----Original Message-----
From: Angelicola,Lisa [mailto:Angelicola@uchc.edu] 
Sent: Monday, August 09, 2004 2:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] F4/80 antibody

I'm working on a research project that involves demonstrating
macrophages in formalin fixed paraffin embedded mouse skin wounds. The
researcher has purchased the F4/80 rat-anti-mouse antibody from abcam.
Has anyone out there in histoland had any experience working on fixed,
processed tissue with this antibody? The spec sheet states that it will
work with pretreatment of the tissue (protein digestion, which I would
like to avoid if possible since the tissue is susceptible to falling
off). Also where do you purchase your anti-rat secondary from? I would
appreciate any advice anyone might have as far as pretreatment,
dilutions, ect.
 
Thanks in advance,
 
Lisa Angelicola
Immunohistochemistry Lab
UCONN Health Center
Farmington, Ct.

 

 

 

 

 

 

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