RE: [Histonet] Diastase dilemma
Are you running a plain PAS on the patient as well? I was always taught
to always run a PAS and PASD even if the Dr only wanted a PASD. It
serves as a control.
If you are doing that, and know there is no diference digested vs
undigested in the patient then I don't know what to say. I would
normally look to something in the tissue that is causing this.
If you were still using spit to digest I would blame it on you eating
sugar before spitting on the slide, or not drinking enough coffee.
Ross M Stapf
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
[mailto:email@example.com] On Behalf Of Angela
Sent: Friday, August 13, 2004 2:05 PM
Subject: [Histonet] Diastase dilemma
Bizarre things happen here all the time, but this is REALLY BIZARRE!
We'll do a PAS w/ diastase and our control tissue will be digested but
the patient tissue won't be. They are mounted on the same slide!
The next day we will run the stain again and we tried turning the slide
upside-down in the Coplin jar. Same problem. We mixed fresh diastase
solution and tried again. Same problem. Tomorrow, we may run it again
and it will work fine. What could be going on? I'm perplexed!
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged.
It is intended solely for the addressee. Access to this message by
anyone else is unauthorized. If you are not the intended recipient, any
disclosure, copying, distribution or any action taken, or omitted to be
taken, in reliance on it is prohibited and may be unlawful. If you have
received this message in error, please delete all electronic copies of
this message (and the documents attached to it, if any), destroy any
hard copies you may have created and notify me immediately by replying
to this email. Thank you.
Histonet mailing list
This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at firstname.lastname@example.org. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information.
Histonet mailing list
<< Previous Message | Next Message >>