Are you running a plain PAS on the patient as well?  I was always taught
to always run a PAS and PASD even if the Dr only wanted a PASD.  It
serves as a control.

If you are doing that, and know there is no diference digested vs
undigested in the patient then I don't know what to say.  I would
normally look to something in the tissue that is causing this.

If you were still using spit to digest I would blame it on you eating
sugar before spitting on the slide, or not drinking enough coffee.

Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465

214-820-4110 fax
RossS@baylorhealth.edu



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Friday, August 13, 2004 2:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Diastase dilemma


Bizarre things happen here all the time, but this is REALLY BIZARRE!
We'll do a PAS w/ diastase and our control tissue will be digested but
the patient tissue won't be. They are mounted on the same slide!

The next day we will run the stain again and we tried turning the slide
upside-down in the Coplin jar. Same problem. We mixed fresh diastase
solution and tried again. Same problem. Tomorrow, we may run it again
and it will work fine. What could be going on? I'm perplexed!

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916


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