[Histonet] IHC artefact puzzle
If you are using heat retrieval with slides in a vertical rack and you
remove the rack from the hot retrieval solution , the solution will evaporate from
the edges of the section first, drying the tissue edges and ruining the
reactivity on the tissue edges. If you leave the racks in the hot solution vessel
after the time is up for retrieval and cool your retrieval solution, racks and
all, by sitting them in a cool water bath without removing them from the
solution until cool (less than 40C) the evaporation will be slowed, the edges will
not dry out, and antigenicity should be preserved. JPJC HT,QIHC
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