[Histonet] HTL Practical-Mallory's PTAH
Okay, so here's my question. Is there a secret to getting a decent section
of longitudinal muscle? I fixed my fresh muscle in Zenker's fluid for about
18 hrs., washed in many changes of water for about 6 hrs., and processed
starting in 70% alcohol (skipping formalin). I embedded the pieces at a 45
degree angle. The sections I'm getting are terrible, all shredded and torn.
I've tried soaking on a wet ice cube and soaking in ammonia water, but
neither seems to help.
Any suggestions would be greatly appreciated!
Brianna Jackson, BS, QIHC
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