[Histonet] Dephosphorylation in FFPE sections

From:"MacDougall, John"

Dear Histonet
	We are using an anti-receptor tyrosine kinase phosphospecific
antibody with good success in FFPE IHC experiments.  We wanted to
confirm our staining by removing the phosphate and hopefully seeing the
staining disappear.  Has anyone performed this type of reaction in FFPE
sections?  If so, please share protocols.

Thanks for any help.

John R. MacDougall, Ph.D.
Project Leader
Preclinical Development - Toxicology/Pathology
CuraGen Corporation
322 East Main St.
Branford, CT, 06405

Voice: 203-871-4320
Fax: 203-315-2735




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