[Histonet] BRDU free floating vibratome sections

From:"Kimberle M. Jacobs"

Hi I have searched the archives and found a lot of discussion of BRDU, but 
no answer to the question if there is a protocol out there for 
formaldehyde-fixed, vibratome-cut, free floating sections.  Actually we 
could mount them on slides as well, I just don't want to do the whole 
paraffin embedding thing.
Also is it really necessary to have thin sections if you are going to use 
pepsin and acid, etc. ??

We could probably cut as thin as 20 um, but would prefer to use 40 or 60 um.

We will be doing this in pregnant ferrets (E36, gestation is ~42 days) and 
examining the brains at ages P0-P15.

Thanks very much for any help!!

Kimberle M. Jacobs, Ph.D.
Assistant Professor
Department of Anatomy and Neurobiology
Virginia Commonwealth University
P.O. Box 980709
Richmond, VA  23298-0709
(804) 827-2135
FAX: (804) 828-9477

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