Re: [Histonet] warthin starry stain
It's probably because the doctor/nurse is letting
the biopsy dry out before they place it in formalin. Even setting a minute on a
pad will do this. We've seen this also with kidney biopsies.
No way to correct it after the fact. No way to make
the W/S stain work. The tissue HAS to be placed in the fixative
IMMEDIATELY.
You might try the Giemsa or Diff-Quik stain.
Sometimes they will still stain the H. pylorii after this drying
out.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont
Hospital
Royal Oak, MI 48073
----- Original Message -----
Sent: Friday, August 22, 2003 6:28
PM
Subject: [Histonet] warthin starry
stain
I am having some trouble with our Warthin-Starry
stain. The peripheri of the gastric biopsy is not getting stained.
The rest of the specimen is well stained. I am not sure if I need to
lengthen the impregnation time or the developer time. I have never had
this happen before. Any suggestions would be greatly
appreciated.
Celia, HT Sioux Valley
Hospital
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