Re: [Histonet] warthin starry stain

From:"Lee & Peggy Wenk"

It's probably because the doctor/nurse is letting the biopsy dry out before they place it in formalin. Even setting a minute on a pad will do this. We've seen this also with kidney biopsies.
 
No way to correct it after the fact. No way to make the W/S stain work. The tissue HAS to be placed in the fixative IMMEDIATELY.
 
You might try the Giemsa or Diff-Quik stain. Sometimes they will still stain the H. pylorii after this drying out.
 
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
----- Original Message -----
From: celia
To: histonet@pathology.swmed.edu
Sent: Friday, August 22, 2003 6:28 PM
Subject: [Histonet] warthin starry stain

I am having some trouble with our Warthin-Starry stain.  The peripheri of the gastric biopsy is not getting stained.  The rest of the specimen is well stained.  I am not sure if I need to lengthen the impregnation time or the developer time.  I have never had this happen before.  Any suggestions would be greatly appreciated.
Celia, HT Sioux Valley Hospital

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