Re: [Histonet] cutting MMA at 0.5 microns

From:"Sarah Jones"

This is for a researcher who is doing apoptag staining. (PMID: 9922213) The reference was for staining in GMA and I thought he might get better results using MMA since the methacrylate can be dissolved away.  I think the reason he needs them so thin is to do the cell identification.     Sarah

>>> Phillip Huff  08/18/03 01:40PM >>>
Just out of curiosity, why do you need your sections so thin? Perhaps you
could cut the sections a bit thicker, and use permeabolization methods or
other methodologies for your staining. BTW, what methodologies will you be
employing on your sections?


I'm attempting to cut horse testis embedded in MMA at 0.5 microns.  The
> problem I'm running into is terrible folds in the section.  I've tried a
> dry knife and a wet knife without any luck.  I'm using MMA kit 14520
> from EMS.  Any help would be appreciated. Thanks, Sarah
> Sarah Jones HT(ASCP)
> Dept. of Vet. Anatomy & Public Health
> Histology Lab
> Texas A&M University
> College Station, TX 77843-4458
> phone: 979-845-3177
> fax:  979-458-3499
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