Re: [Histonet] cutting MMA at 0.5 microns

From:Phillip Huff

Just out of curiosity, why do you need your sections so thin? Perhaps you
could cut the sections a bit thicker, and use permeabolization methods or
other methodologies for your staining. BTW, what methodologies will you be
employing on your sections?


I'm attempting to cut horse testis embedded in MMA at 0.5 microns.  The
> problem I'm running into is terrible folds in the section.  I've tried a
> dry knife and a wet knife without any luck.  I'm using MMA kit 14520
> from EMS.  Any help would be appreciated. Thanks, Sarah
> Sarah Jones HT(ASCP)
> Dept. of Vet. Anatomy & Public Health
> Histology Lab
> Texas A&M University
> College Station, TX 77843-4458
> phone: 979-845-3177
> fax:  979-458-3499
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