Re: [Histonet] Processor failure
I hope you are having luck rehydrating the BB's. Just a thought and I don't
have the procedure at hand but some time ago there was an article (or maybe
workshop) that included a way to hydrate old museum specimens and parts of
mummies. Maybe somebody knows about this?
There is also something called Ruffer's solution or Zimmerman's that is a
combination of 50 parts water, 30 parts absolute alcohol and 20 parts of a
5% Aqueous Sodium Carbonate. Page 33, Freida Carson's book, 2nd edition.
Good luck!! Send us a e-postcard from Bora Bora.
At 06:36 AM 8/28/2003 -0500, you wrote:
>Sounds great - however I'm writing this from my laptop on a direct flight
>from Chicago to Bora Bora. I've brought the BB's along with me to soak in
>South Pacific seawater (saline). Thanks for the offer,
>however. Charleston will sound a lot more enticing around January in Chicago.
>"Vinnie Della Speranza"
>08/27/2003 03:39 PM
> To: ,
> Subject: Re: [Histonet] Processor failure
>I think I've got an easier solution for you.
>pack an overnight bag and start driving toward Charleston (you won't need
>much in the way of clothing here) we'll find a place for you in the lab
>here and those little BBs will be someone else's headache. by the time
>they realize you're gone you'll already be far enough away that you won't
>have to hear the crying and whining.
>thought you should at least consider ALL of your options.
>Hey sometimes I find myself fantasizing about running away but then I
>realize I am already here in Charleston and where else would I go???
> >>> 08/27/03 08:28AM >>>
>I haven't done this in years. I came in this a.m. to find the tissues on
>my processor suspended in air over the first absolute alcohol station.
>Apparently, the machine, a Shandon Citadel 2000, was moved too close to a
>wall and stuck as it tried to rotate. I have about 100 xenografts to
>rescue. Right now they all look and feel like bb's. My plan is to
>rehydrate them and reprocess them, but I have my doubts as to the quality
>of the morphology and any subsequent IHC. As I said, I haven't screwed up
>a basket of tissue in years and years - so I would appreciate any new
>ideas on possibly saving these tumors. Thanks in advance.
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: firstname.lastname@example.org) :
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