Go to http://members.pgonline/~bryand/StainsFile/stain/cell/aldtolblue.htm.
This method should do what you want.

Bryan Llewellyn


----- Original Message -----
From: 
To: 
Sent: Friday, August 29, 2003 1:26 PM
Subject: [Histonet] Mast vs eosinophils


> Hi everyone,
>
> I need input on determining between mast and eosinophils. I am staining
> formalin fixed paraffin embedded human scar tissue with acidified
> tol-blue-0 (ph 4). I keep getting increased numbers of violet marked cells
> that I believe are eosinophils, and I only want mast cells with intact
> granulation.
>
> Any suggestions?
>
> _cheers,
>
> Rebecca Armendariz
> Harborview Medical Center
> Univerisity of Washington, Seattle
> rtarmen@drizzle.com
>
>
>
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