Re: [Histonet] Marine histology

From:"Vinnie Della Speranza"

I recommend that you contact Marie Wright at  the Gulf Coast Research Laboratories of the Univ of Mississippi. She has extensive experience with crustaceans. 

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974

>>> Kathleen Roberts  08/20/03 10:03AM >>>
Good morning Histonetters-

I just got a seahorse from one of our university vets-he wants it
processed & stained with H& E.  It was fixed in Formalde-fresh buffered
formalin for nine days, and it needs to be decalcified.  Would anybody
happen to know how long it would take a seahorse to decalcify?  I've
done fish before, but not seahorses.  Any advice on processing would be
welcome as well.

(Yes, I know about the book Dr. Callis wrote on animal tissue
processing; it hasn't gotten here yet!  :o)

Thanks for all your help-
Kathleen Roberts
Principal Lab Technician
Molecular Pathology Core Facility
Dept of Pharmacology & Toxicology
Ernest Mario School of Pharmacy
Rutgers University
41 B Gordon Rd
Piscataway, NJ 08854

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