RE: [Histonet] reagent alcohol

From:"Nick Kirk"


We stopped using absolute ethanol many years ago as the extra duty (tax) you
had to pay on it made it very expensive for the volumes we used here in the
We don't even use a graduated series of alcohols on our processing machines
anymore, just straight from Formalin to Industrial methylated spirits (IMS)
which is around 99+% ethanol with a bit of methanol in it (means you can't
make gin out of it but we all have our sacrifices to bear).
We use this on all our routine processing and have never had any problems at
all. I know a lot of the labs in the UK use the same protocol.
The tissues then go straight into Xylene to clear and then wax. We don't do
a lot of bone work, but the femoral heads and marrow trephines we process on
it never seem to suffer.

Nick Kirk
Head BMS
Hinchingbrooke Hospital

-----Original Message-----
[]On Behalf Of DiCarlo,
Sent: 19 August 2003 19:15
To: ''
Subject: [Histonet] reagent alcohol


I have both 95% and absolute ethyl alcohol. A resident gave me some reagent
absolute alcohol to use for his project.  I need to know if I can
interchange reagent with ethanol for processing and staining.  Any
information will be greatly appreciated.

Peggy DiCarlo HT (ASCP)
Ortho Bone Lab
Buffalo General Hospital

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