Hi Jackie.  I would recommend rehydrating them in normal saline
instead of water.  Make certain that the samples are adequately
rehydrated before processing again.
 
Fred

	-----Original Message-----
From:  
Sent: Wednesday, August 27, 2003 8:28 AM
To: 
Subject: [Histonet] Processor failure



	Fellow histonetters: 
I haven't done this in years.  I came in this a.m. to find the
tissues on 
my processor suspended in air over the first absolute alcohol station.

Apparently, the machine, a Shandon Citadel 2000, was moved too close to
a 
wall and stuck as it tried to rotate.  I have about 100 xenografts to

rescue.  Right now they all look and feel like bb's.  My plan is to

rehydrate them and reprocess them, but I have my doubts as to the
quality 
of the morphology and any subsequent IHC.  As I said, I haven't
screwed up 
a basket of tissue in years and years   so I would appreciate any new

ideas on possibly saving these tumors.  Thanks in advance. 

	Jackie O'Connor 


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