RE: [Histonet] Processor failure

From:"Favara, Cynthia (NIH/NIAID)"

I had this happen repeatedly and eventually found out the rotator was bad on the processor so be aware of further problems. The rotator on my processor would rotate at will and plunk in any basket.
To rescue the tissue I would just place back in the solution it was in before suspended in air then proceed with the processing. I would mostly with mouse tissue and samples were readable but we elected to repeat our studies as the morphology was less than perfect and cutting was a nightmare. Not hopeless just difficult!

Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

-----Original Message-----
From: Jackie.O' [mailto:Jackie.O']
Sent: Wednesday, August 27, 2003 6:28 AM
Subject: [Histonet] Processor failure

Fellow histonetters:
I haven't done this in years.  I came in this a.m. to find the tissues on my processor suspended in air over the first absolute alcohol station.  Apparently, the machine, a Shandon Citadel 2000, was moved too close to a wall and stuck as it tried to rotate.  I have about 100 xenografts to rescue.  Right now they all look and feel like bb's.  My plan is to rehydrate them and reprocess them, but I have my doubts as to the quality of the morphology and any subsequent IHC.  As I said, I haven't screwed up a basket of tissue in years and years - so I would appreciate any new ideas on possibly saving these tumors.  Thanks in advance.

Jackie O'Connor

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