|From:||"Favara, Cynthia (NIH/NIAID)" |
903 South 4th Street
Hamilton, MT 59840
From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com]
Sent: Wednesday, August 27, 2003 6:28 AM
Subject: [Histonet] Processor failure
I haven't done this in years. I came in this a.m. to find the tissues on my processor suspended in air over the first absolute alcohol station. Apparently, the machine, a Shandon Citadel 2000, was moved too close to a wall and stuck as it tried to rotate. I have about 100 xenografts to rescue. Right now they all look and feel like bb's. My plan is to rehydrate them and reprocess them, but I have my doubts as to the quality of the morphology and any subsequent IHC. As I said, I haven't screwed up a basket of tissue in years and years - so I would appreciate any new ideas on possibly saving these tumors. Thanks in advance.