| From: | "Morken, Tim - Labvision" |
Majid asks: "if your primary antibody is a mouse IgM, by using a secondary anti-mouse IgG
are you running any risk of getting artefactual signals?"
Yes, your results will be negative. The anti-IgG will not detect the IgM
primary antibody.
Tim
Morken
Lab
Vision / NeoMarkers
www.labvision.com
-----Original Message-----
From: Majid Ghoddusi
[mailto:mghoddusi@cmri.usyd.edu.au]
Sent: Monday, August 25, 2003
12:21 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] IHC- using IgM
vs. IgG
Hello dear histonetters
Just a very basic question for IHC
experts:
if your primary antibody is a mouse
IgM, by using a secondary anti-mouse IgG are you running any risk of
getting artefactual signals?
Thanks very much in advance for any
help and advice including pointing to a reference that might discuss this
in detail.
...........................................................
Majid Ghoddusi
Children's Medical Research
Institute
Locked Bag 23, Wentworthville NSW 2145
Tel: (02) 9687-2800
Fax:(02) 9687-2120
.............................................................