RE: [Histonet] Formalin blues

From:"Mandy Townsend"

Serotec are able to supply an antibody against human CD31 that is suitable for IHC on paraffin sections, requiring HIER with Citrate, clone number 1A10. This is also available in a smaller sample size for evaluation.  Please contact me if you require any further information.


Mandy Townsend MSc
Technical Services Supervisor
Serotec Ltd
22 Bankside
Station Approach

Tel: +44 1865 852736
Fax: +44 1865 852739
Serotec-Your first choice for antibodies!

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-----Original Message-----
From: Christian Franci []
Sent: Monday, August 25, 2003 6:31 PM
Subject: [Histonet] Formalin blues

I'm studying neo-vascularization.
It seems that the only antibodies to CD31 (murine and Human) that I can find
commercially (BD Bio and Chemicon) do not work at all for formalin fixed,
paraffin imbedded tissue sections.

Does anyone out there know of any anti CD31 Ab's that DO work in formalin
fixed tissues? 

Also, does anyone know of any Ab that I could use as a positive control (for
both mouse and human tissues) that works well in formalin-fixed samples?

Lastly, does anyone have a better fixation method to formalin (compatible
with paraffin imbedding) which may allow me to use the above mentioned
commercial Ab's?

Thanks for your help!

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