[Histonet] RE: formalin blues / mouse CD31 IHC
|From:||"PALMER Jason (SVHM)" |
I'm also trying to get an anti mouse CD31 to work on FFPE sections (CBL 1337 from Cymbus / Chemicon). Has anybody had success of any sort with this antibody and type of tissue, and if so, what's your secret? (By the the way, Dako anti human CD31 (M 0823) works just fine on human FFPE tissues for me).
Bernard O'Brien Institute of Microsurgery
Date: Mon, 25 Aug 2003 10:30:55 -0700
From: Christian Franci
Subject: [Histonet] Formalin blues
I'm studying neo-vascularization.
It seems that the only antibodies to CD31 (murine and Human) that I can find
commercially (BD Bio and Chemicon) do not work at all for formalin fixed,
paraffin imbedded tissue sections.
Does anyone out there know of any anti CD31 Ab's that DO work in formalin
Also, does anyone know of any Ab that I could use as a positive control (for
both mouse and human tissues) that works well in formalin-fixed samples?
Lastly, does anyone have a better fixation method to formalin (compatible
with paraffin imbedding) which may allow me to use the above mentioned
Thanks for your help!
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