[Histonet] RE: formalin blues / mouse CD31 IHC

From:"PALMER Jason (SVHM)"


	Hi all.
	I'm also trying to get an anti mouse CD31 to work on FFPE sections (CBL 1337 from Cymbus / Chemicon). Has anybody had success of any sort with this antibody and type of tissue, and if so, what's your secret? (By the the way, Dako anti human CD31 (M 0823) works just fine on human FFPE tissues for me).
	Much obliged!
	Jason Palmer
	Bernard O'Brien Institute of Microsurgery
	Melbourne, Australia

		Date: Mon, 25 Aug 2003 10:30:55 -0700
		From: Christian Franci 
		Subject: [Histonet] Formalin blues
		I'm studying neo-vascularization.
		It seems that the only antibodies to CD31 (murine and Human) that I can find
		commercially (BD Bio and Chemicon) do not work at all for formalin fixed,
		paraffin imbedded tissue sections.
		Does anyone out there know of any anti CD31 Ab's that DO work in formalin
		fixed tissues?
		Also, does anyone know of any Ab that I could use as a positive control (for
		both mouse and human tissues) that works well in formalin-fixed samples?
		Lastly, does anyone have a better fixation method to formalin (compatible
		with paraffin imbedding) which may allow me to use the above mentioned
		commercial Ab's?
		Thanks for your help!


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