[Histonet] RE: IHC- using IgM vs. IgG

From:"C.M. van der Loos"

Dear Majid,
To my knowledge secondary antibodies aren't just anti-IgG 
specific rather than "anti-immunoglobulins" specific. This includes 
that whenever a vendor uses the term "anti-immunoglobulin" with respect 
to a secondary antibody reagent (no matter the label) there should be a 
specificity against the heavy + light chain of all immunoglobulin 
isotypes IgG, IgM, etc. of the indicated species. Unless stated by the 
vendor that a secondary antibody is for example anti-IgG heavy chain 
specific. In those cases there will be no reaction with a mouse IgM 
primary antibody. 
So far I failed in finding some literature references about this. 
Somebody else perhaps? I would like to have it too....

Chris van der Loos
Dept. of Cardiovascular Pathology
Academical Medical Center H0-120
Meibergdreef 9
NL-1105 AZ Amsterdam - The Netherlands  

>From: Majid Ghoddusi 
>To: "'histonet@lists.utsouthwestern.edu'" 
>Date: Mon, 25 Aug 2003 17:21:26 +1000
>Subject: [Histonet] IHC- using IgM vs. IgG
>Hello dear histonetters
>Just a very basic question for IHC experts:
>if your primary antibody is a mouse IgM, by using a secondary anti-
>mouse IgG are you running any risk  of getting artefactual signals?
>Thanks very much in advance for any help and advice including pointing 
>to a reference that might discuss this in detail.
>Majid Ghoddusi 
>Children's Medical Research Institute
>Locked Bag 23, Wentworthville NSW 2145

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