[Histonet] PASM problem

From:"Elizabeth Chlipala"

Hello All


We have been working on the Jones stain for kidney basement membranes.  We have our internal control specimen which is a human specimen.  The other specimens we are staining are rat kidneys.  When we stain the slides, the human kidney comes out great, very good staining of the basement membranes, very slight nuclear staining with no precipitate.  The rat kidneys don’t look as nice, there is good staining of the basement membrane, but the nuclear staining is more predominant and there is intense granular staining in the cytoplasm, which sometimes looks like a precipitate.  Does anyone have any suggestions?  Thanks in advance.




Elizabeth A. Chlipala, BS, HTL(ASCP)

Premier Histology Laboratory, LLC

P.O. Box 18592

Boulder, Colorado 80308

Office: (303) 735-5001

Fax: (303) 735-3540




Ship to Address:

Premier Histology Laboratory

University of Colorado

MCBD, Room A3B40

Boulder, Colorado 80309


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