[Histonet] Orcein Variation for EVG to detect Elastosis Perforans Serpiginosum

From:"kevin williams"

Dear MT( and all you HTs out there):

Thanks for the information lots of help:

I am now on the quest for a procedure for Elastosis Perforans Serpiginosum.

My pathologist has asked for the Orcein variation on the EVG(Elastin Van Gieson) or VVG as some of you know it. I have to get the Orcein so that is my first challenge, second is my protocol. There are a few out there and I would like to here from anyone who has a favorite for this method with this disease process.

Yours faithfully A. Keivn Williams

>From: MTitford@aol.com
>To: Histonet@lists.utsouthwestern.edu
>Subject: [Histonet] Alpha Amylase
>Date: Fri, 29 Aug 2003 15:50:51 -0400
>Robert Lotts asks about amylase:
>We use Sigma Alpha amylase Cat # A-3176.(0.125% at 37 degrees centigrade for 30 minutes). I zap it in the microwave to get it up to temperature
>Mike Titford
>USA Pathology
>Mobile AL 36617 USA
>Histonet mailing list

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