[Histonet] Mast vs eosinophils

From:

Hi everyone,

I need input on determining between mast and eosinophils. I am staining
formalin fixed paraffin embedded human scar tissue with acidified
tol-blue-0 (ph 4). I keep getting increased numbers of violet marked cells
that I believe are eosinophils, and I only want mast cells with intact
granulation.

Any suggestions?

_cheers,

Rebecca Armendariz
Harborview Medical Center
Univerisity of Washington, Seattle
rtarmen@drizzle.com



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